After the start button is pressed HD Tune Pro will perform several tests to measure the performance of the hard disk.
Transfer rate test
The data transfer rate is measured accross the entire disk (0% to 100% of the disk's capacity). The unit of measurement is MB/s (1 MB = 1024 KB = 1024 bytes).
Both read and write transfer rate can be measured. To prevent accidental data loss, the write test can only be performed on a disk with no partitions.
To remove all partitions go to Control Panel -> Administrative Tools -> Computer Management -> Disk Management, right-click on the partitions on the hard disk you want to test and select delete partition.
A newly bought hard disk usually doesn't have any partitions yet so this step is not necessary to run the write test.
WARNING: RUNNING THE WRITE TEST OR REMOVING PARTITIONS WILL REMOVE DATA FROM THE DISK.
Access Time
The average access time is measured and displayed in milli-seconds (ms). The measured access times are shown on the graph as the yellow dots.
Burst Rate
The burst rate is the highest speed (in megabytes per second) at which data can be transferred from the drive interface (IDE or SCSI for example) to the operating system.
CPU Usage
The CPU usage shows how much CPU time (in %) the system needs to read data from the hard disk.
screenshots

read test

write test
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